27 April 2023

We are pleased to inform you know that a new article about h-ALO project has been published as open access – Advanced Materials – on CNR-ISMN and CNR websites.

“A fully integrated miniaturized optical biosensor for fast and multiplexing plasmonic detection of high- and low-molecular weight analytes”

Abstract

Optical biosensors based on plasmonic sensing schemes combine high sensitivity and selectivity with label-free detection. However, the use of bulky optical components is still hampering the possibility of obtaining miniaturized systems required for analysis in real settings. Here, we demonstrate a fully miniaturized optical biosensor prototype based on plasmonic detection that enables fast and multiplex sensing of analytes with high- and low-molecular weight (80 000 and 582 Dalton) as quality and safety parameters for milk: a protein (lactoferrin) and an antibiotic (streptomycin). The optical sensor is based on the smart integration of (i) miniatuirized organic optoelectronic devices used as light-emitting and -sensing elements and (ii) a functionalized nanostructured plasmonic grating for highly sensitive and specific localized surface plasmon resonance (SPR) detection. The sensor provides quantitative and linear response reaching a limit of detection of 10−4 refractive index units once it is calibrated by standard solutions. Analyte-specific and rapid (15 minute-long) immunoassay-based detection was demonstrated for both targets. By using a custom algorithm based on principal-component analysis, a linear dose-response curve was constructed which correlated with a limit of detection (LOD) as low as 3.7 μg/mL for lactoferrin, thus assensing the miniaturized optical biosensor well-alined with the chosen reference benchtop SPR method.

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